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Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit

Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit

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Specification

Product name Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit employs a two-site sandwich ELISA to quantitate PON2 in samples. An antibody specific for PON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPON2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PON2; A-esterase 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2

Product Properties

Kit components • Mouse Serum paraoxonase/arylesterase 2 microplate
• Mouse Serum paraoxonase/arylesterase 2 standard
• Mouse Serum paraoxonase/arylesterase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serum paraoxonase/arylesterase 2 (PON2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PON2. No significant cross-reactivity or interference between Mouse PON2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Serum paraoxonase/arylesterase 2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID 330260
Alternative PON2; A-esterase 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2
Accession Q62086

Image & description

Fig.1. Mouse Serum paraoxonase/arylesterase 2 (PON2) Standard Curve.

Fig.1. Mouse Serum paraoxonase/arylesterase 2 (PON2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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