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Mouse Serum amyloid A2 (SAA2) ELISA Kit

Mouse Serum amyloid A2 (SAA2) ELISA Kit

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Product name Mouse Serum amyloid A2 (SAA2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serum amyloid A2 (SAA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SAA2 in samples. An antibody specific for SAA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySAA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SAA2

Product Properties

Kit components • Mouse Serum amyloid A2 microplate
• Mouse Serum amyloid A2 standard
• Mouse Serum amyloid A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serum amyloid A2 (SAA2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SAA2. No significant cross-reactivity or interference between Mouse SAA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The 6 sequences, referred to as SAA1, SAA1 des Arg, SAA2-alpha, SAA2-alpha des Arg, SAA2-beta, and SAA2-beta des Arg, are resolved by electrofocusing into 6 isoforms. SAA1 is the product of one gene, while SAA2-alpha and SAA2-beta, which differ by only a single amino acid, represent allelic sequences of a second gene. Although the first reported AA protein corresponded to SAA2-beta, subsequent analyses showed that the vast majority of the protein isolated from amyloid deposits is derived from SAA1. The SAA2-tat/HIV-sTNFR:Ig construct, and derivatives thereof, may therefore be ideally suited to gene therapy applications that require the local production of potent and specific immune modifiers only when there is active pathology..
Gene ID 20209
Alternative SAA2
Accession P05367

Image & description

Fig.1. Mouse Serum amyloid A2 (SAA2) Standard Curve.

Fig.1. Mouse Serum amyloid A2 (SAA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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