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Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit

Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit

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Specification

Product name Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1CB in samples. An antibody specific for PPP1CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PPP1CB; MGC3672; PP-1B; PPP1CD; OTTHUMP00000200968; protein phosphatase 1; catalytic subunit; beta; protein phosphatase 1; catalytic subunit; delta isoform; protein phosphatase 1-beta; protein phosphatase 1-del

Product Properties

Kit components • Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit microplate
• Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit standard
• Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPP1CB. No significant cross-reactivity or interference between Mouse PPP1CB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. PP1 has 3 catalytic subunits, designated alpha, beta, and gamma. PP1-beta is also referred to as PP1-delta. PPP1Cb is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory.
Gene ID 19046
Alternative PPP1CB; MGC3672; PP-1B; PPP1CD; OTTHUMP00000200968; protein phosphatase 1; catalytic subunit; beta; protein phosphatase 1; catalytic subunit; delta isoform; protein phosphatase 1-beta; protein phosphatase 1-del
Accession P62141

Image & description

Fig.1. Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) Standard Curve.

Fig.1. Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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