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Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit

Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit

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Specification

Product name Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIM2 in samples. An antibody specific for PIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIM2; Proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2

Product Properties

Kit components • Mouse Serine/threonine-protein kinase pim-2 microplate
• Mouse Serine/threonine-protein kinase pim-2 standard
• Mouse Serine/threonine-protein kinase pim-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIM2. No significant cross-reactivity or interference between Mouse PIM2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PIM2 encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival. The deduced 334-amino acid sequence of the clone showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts. A 2.2-kb transcript was expressed in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. A 5.0-kb transcript was expressed only in spleen, thymus, small intestine, colon, and in very small amounts in peripheral blood leukocytes.
Gene ID 18715
Alternative PIM2; Proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2
Accession Q62070

Image & description

Fig.1. Mouse Serine/threonine-protein kinase pim-2 (PIM2) Standard Curve.

Fig.1. Mouse Serine/threonine-protein kinase pim-2 (PIM2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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