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Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit

Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit

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Specification

Product name Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK3 in samples. An antibody specific for DCLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DCLK3; CLR; DCAMKL3; DCDC3C; DCK3; KIAA1765; doublecortin and CaM kinase-like 3

Product Properties

Kit components • Mouse Serine/threonine-protein kinase DCLK3 microplate
• Mouse Serine/threonine-protein kinase DCLK3 standard
• Mouse Serine/threonine-protein kinase DCLK3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DCLK3. No significant cross-reactivity or interference between Mouse DCLK3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Doublecortin-like kinase 3 (Dclk3) encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. 
Gene ID 245038
Alternative DCLK3; CLR; DCAMKL3; DCDC3C; DCK3; KIAA1765; doublecortin and CaM kinase-like 3
Accession Q8BWQ5

Image & description

Fig.1. Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) Standard Curve.

Fig.1. Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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