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Mouse Selenoprotein P (SEPP1) ELISA Kit

Mouse Selenoprotein P (SEPP1) ELISA Kit

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Specification

Product name Mouse Selenoprotein P (SEPP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Selenoprotein P (SEPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPP1 in samples. An antibody specific for SEPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEPP1; SELP; SeP; selenoprotein P

Product Properties

Kit components • Mouse Selenoprotein P microplate
• Mouse Selenoprotein P standard
• Mouse Selenoprotein P detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Selenoprotein P (SEPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SEPP1. No significant cross-reactivity or interference between Mouse SEPP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Selenoprotein P is a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene.
Gene ID 20363
Alternative SEPP1; SELP; SeP; selenoprotein P
Accession P70274

Image & description

Fig.1. Mouse Selenoprotein P (SEPP1) Standard Curve.

Fig.1. Mouse Selenoprotein P (SEPP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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