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Mouse Retinol-binding protein 4 (RBP4) ELISA Kit

Mouse Retinol-binding protein 4 (RBP4) ELISA Kit

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Specification

Product name Mouse Retinol-binding protein 4 (RBP4) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Retinol-binding protein 4 (RBP4) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP4 in samples. An antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RBP4; OTTHUMP00000020116; retinol-binding protein 4; interstitial; retinol-binding protein 4; plasma

Product Properties

Kit components • Mouse Retinol-binding protein 4 microplate
• Mouse Retinol-binding protein 4 standard
• Mouse Retinol-binding protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Retinol-binding protein 4 (RBP4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RBP4. No significant cross-reactivity or interference between Mouse RBP4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Yang et al. noted that adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders.
Gene ID 19662
Alternative RBP4; OTTHUMP00000020116; retinol-binding protein 4; interstitial; retinol-binding protein 4; plasma
Accession Q00724

Image & description

Fig.1. Mouse Retinol-binding protein 4 (RBP4) Standard Curve.

Fig.1. Mouse Retinol-binding protein 4 (RBP4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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