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Mouse Putative malate dehydrogenase 1B (MDH1B) ELISA Kit

Mouse Putative malate dehydrogenase 1B (MDH1B) ELISA Kit

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Specification

Product name Mouse Putative malate dehydrogenase 1B (MDH1B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Putative malate dehydrogenase 1B (MDH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MDH1B in samples. An antibody specific for MDH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MDH1B; FLJ25341; RP11-95H11;

Product Properties

Kit components • Mouse Putative malate dehydrogenase 1B microplate
• Mouse Putative malate dehydrogenase 1B standard
• Mouse Putative malate dehydrogenase 1B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Putative malate dehydrogenase 1B (MDH1B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MDH1B. No significant cross-reactivity or interference between Mouse MDH1B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MDH1B (Malate Dehydrogenase 1B) is a Protein Coding gene. GO annotations related to this gene include oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor and malate dehydrogenase activity.
Gene ID 76668
Alternative MDH1B; FLJ25341; RP11-95H11;
Accession Q5F204

Image & description

Fig.1. Mouse Putative malate dehydrogenase 1B (MDH1B) Standard Curve.

Fig.1. Mouse Putative malate dehydrogenase 1B (MDH1B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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