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Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit

Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit

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Specification

Product name Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit employs a two-site sandwich ELISA to quantitate PPOX in samples. An antibody specific for PPOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PPOX; MGC8485; PPO; V290M; VP;

Product Properties

Kit components • Mouse Protoporphyrinogen oxidase microplate
• Mouse Protoporphyrinogen oxidase standard
• Mouse Protoporphyrinogen oxidase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPOX. No significant cross-reactivity or interference between Mouse PPOX and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PPOX is a human gene that produces an enzyme called protoporphyrinogen oxidase. This enzyme is responsible for the seventh step in heme production. Heme is the portion of hemoglobin that carries oxygen in the blood from the lungs to the rest of the body. Each of the steps in heme production is controlled by a separate gene. Protoporphyrinogen oxidase removes hydrogen atoms from protoporphyrinogen IX (the product of the sixth step in the production of heme) to form protoporphyrin IX. One additional enzyme must modify protoporphyrin IX before it becomes heme.This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. This protein is a flavoprotein associated with the outer surface of the inner mitochondrial membrane.
Gene ID 19044
Alternative PPOX; MGC8485; PPO; V290M; VP;
Accession P51175

Image & description

Fig.1. Mouse Protoporphyrinogen oxidase (PPOX) Standard Curve.

Fig.1. Mouse Protoporphyrinogen oxidase (PPOX) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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