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Mouse Protein Mdm4 (MDM4) ELISA Kit

Mouse Protein Mdm4 (MDM4) ELISA Kit

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Specification

Product name Mouse Protein Mdm4 (MDM4) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protein Mdm4 (MDM4) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM4 in samples. An antibody specific for MDM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MDM4; RP11-430C7.1; DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4-related protein 1; Mdm4; transformed 3T3 cell double minute 4; p53 binding protein; double minute 4; human homolog of; p53-binding protei

Product Properties

Kit components • Mouse Protein Mdm4 microplate
• Mouse Protein Mdm4 standard
• Mouse Protein Mdm4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protein Mdm4 (MDM4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MDM4. No significant cross-reactivity or interference between Mouse MDM4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mdm4 encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of Mdm4 or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of Mdm4 results in multiple transcript variants.
Gene ID 17248
Alternative MDM4; RP11-430C7.1; DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4-related protein 1; Mdm4; transformed 3T3 cell double minute 4; p53 binding protein; double minute 4; human homolog of; p53-binding protei
Accession O35618

Image & description

Fig.1. Mouse Protein Mdm4 (MDM4) Standard Curve.

Fig.1. Mouse Protein Mdm4 (MDM4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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