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Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit

Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit

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Specification

Product name Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit employs a two-site sandwich ELISA to quantitate DISP2 in samples. An antibody specific for DISP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DISP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DISP2; DISPB; DKFZp547N223; HsT16908; KIAA1742; dispatched B

Product Properties

Kit components • Mouse Protein dispatched homolog 2 microplate
• Mouse Protein dispatched homolog 2 standard
• Mouse Protein dispatched homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protein dispatched homolog 2 (DISP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DISP2. No significant cross-reactivity or interference between Mouse DISP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched.Ma et al. (2002) isolated cDNAs encoding 2 murine homologs of dipatched, which they called Dispa and Dispb. They predicted that the dispatched proteins all have 12 transmembrane spans with cytoplasmic N and C termini.
Gene ID 214240
Alternative DISP2; DISPB; DKFZp547N223; HsT16908; KIAA1742; dispatched B
Accession Q8CIP5

Image & description

Fig.1. Mouse Protein dispatched homolog 2 (DISP2) Standard Curve.

Fig.1. Mouse Protein dispatched homolog 2 (DISP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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