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Mouse Protein delta homolog 2 (DLK2) ELISA Kit

Mouse Protein delta homolog 2 (DLK2) ELISA Kit

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Specification

Product name Mouse Protein delta homolog 2 (DLK2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protein delta homolog 2 (DLK2) ELISA Kit employs a two-site sandwich ELISA to quantitate DLK2 in samples. An antibody specific for DLK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DLK2; EGFL9; MGC111055; MGC2487; EGF-like-domain; multiple 9

Product Properties

Kit components • Mouse Protein delta homolog 2 microplate
• Mouse Protein delta homolog 2 standard
• Mouse Protein delta homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protein delta homolog 2 (DLK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLK2. No significant cross-reactivity or interference between Mouse DLK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Dlk1 gene appears to function as a regulator of adipogenesis. Adult Dlk1-deficient mice are obese, but adipose tissue still develops in transgenic mice overexpressing an Fc-dlk1 fusion protein, and neither type of genetically modified mice displays serious abnormalities. EGFL9, encoding for a protein whose structural features are virtually identical to those of dlk1, suggesting it may function in a similar way. As dlk1 does, the protein encoded by EGFL9/Dlk2 affects adipogenesis of 3T3-L1 preadipocytes and mesenchymal C3H10T1/2 cells; however, it does so in an opposite way to that of dlk1. In addition, expression levels of both genes appear to be inversely correlated in both cell lines. Moreover, enforced changes in the expression of one gene affect the expression levels of the other.
Gene ID 106565
Alternative DLK2; EGFL9; MGC111055; MGC2487; EGF-like-domain; multiple 9
Accession Q8K1E3

Image & description

Fig.1. Mouse Protein delta homolog 2 (DLK2) Standard Curve.

Fig.1. Mouse Protein delta homolog 2 (DLK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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