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Mouse Protein artemis (DCLRE1C) ELISA Kit

Mouse Protein artemis (DCLRE1C) ELISA Kit

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Specification

Product name Mouse Protein artemis (DCLRE1C) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protein artemis (DCLRE1C) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLRE1C in samples. An antibody specific for DCLRE1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLRE1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLRE1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLRE1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DCLRE1C; RP11-398C13.5; A-SCID; DCLREC1C; FLJ11360; FLJ36438; RS-SCID; SCIDA; SNM1C; OTTHUMP00000019166; OTTHUMP00000019167; OTTHUMP00000019168; OTTHUMP00000019170; OTTHUMP00000019171; OTTHUMP00000019172; artemis

Product Properties

Kit components • Mouse Protein artemis microplate
• Mouse Protein artemis standard
• Mouse Protein artemis detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protein artemis (DCLRE1C) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DCLRE1C. No significant cross-reactivity or interference between Mouse DCLRE1C and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DNA cross-link repair 1C (Dclre1c) encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 227525
Alternative DCLRE1C; RP11-398C13.5; A-SCID; DCLREC1C; FLJ11360; FLJ36438; RS-SCID; SCIDA; SNM1C; OTTHUMP00000019166; OTTHUMP00000019167; OTTHUMP00000019168; OTTHUMP00000019170; OTTHUMP00000019171; OTTHUMP00000019172; artemis
Accession Q8K4J0

Image & description

Fig.1. Mouse Protein artemis (DCLRE1C) Standard Curve.

Fig.1. Mouse Protein artemis (DCLRE1C) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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