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Mouse Protein AF-10 (MLLT10) ELISA Kit

Mouse Protein AF-10 (MLLT10) ELISA Kit

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Specification

Product name Mouse Protein AF-10 (MLLT10) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protein AF-10 (MLLT10) ELISA Kit employs a two-site sandwich ELISA to quantitate MLLT10 in samples. An antibody specific for MLLT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMLLT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLLT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLLT10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MLLT10; RP11-399C16.2; AF10; DKFZp686E10210; MGC75086; ALL-1 fused gene from chromosome 10; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to; 10; myeloid/lymphoid or mixed-linea

Product Properties

Kit components • Mouse Protein AF-10 microplate
• Mouse Protein AF-10 standard
• Mouse Protein AF-10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protein AF-10 (MLLT10) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MLLT10. No significant cross-reactivity or interference between Mouse MLLT10 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The 11q23 gene involved in the translocations is MLL. In both patients, the translocation fused MLL to a gene that the authors designated AF10. The AF10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain that are highly homologous to similar domains in AF17 and BR140. Northern blot analysis showed that AF10 is expressed as a 5.5-kb mRNA in many tissues with highest expression in testis, and has an alternatively spliced exon.all known translocations involving MLL and AF10 or AF17 have involved breakpoints between the zinc finger and leucine zipper domains, and have yielded fusion proteins in which the MLL zinc fingers were replaced with leucine zipper regions.
Gene ID 17354
Alternative MLLT10; RP11-399C16.2; AF10; DKFZp686E10210; MGC75086; ALL-1 fused gene from chromosome 10; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to; 10; myeloid/lymphoid or mixed-linea
Accession O54826

Image & description

Fig.1. Mouse Protein AF-10 (MLLT10) Standard Curve.

Fig.1. Mouse Protein AF-10 (MLLT10) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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