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Mouse Protection of telomeres protein 1 (POT1) ELISA Kit

Mouse Protection of telomeres protein 1 (POT1) ELISA Kit

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Specification

Product name Mouse Protection of telomeres protein 1 (POT1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Protection of telomeres protein 1 (POT1) ELISA Kit employs a two-site sandwich ELISA to quantitate POT1 in samples. An antibody specific for POT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POT1; DKFZp586D211; HPOT1; OTTHUMP00000211676; POT1-like telomere end-binding protein; protection of telomeres 1

Product Properties

Kit components • Mouse Protection of telomeres protein 1 microplate
• Mouse Protection of telomeres protein 1 standard
• Mouse Protection of telomeres protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Protection of telomeres protein 1 (POT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POT1. No significant cross-reactivity or interference between Mouse POT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background POT1 is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.Human POT1 encodes a 71-kD protein whose N-terminal region shares 26% identity with the N terminus of S. pombe Pot1. POT1 is a housekeeping gene required to ensure the integrity of chromosome ends in all cells.
Gene ID 101185
Alternative POT1; DKFZp586D211; HPOT1; OTTHUMP00000211676; POT1-like telomere end-binding protein; protection of telomeres 1
Accession Q91WC1

Image & description

Fig.1. Mouse Protection of telomeres protein 1 (POT1) Standard Curve.

Fig.1. Mouse Protection of telomeres protein 1 (POT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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