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Mouse Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit

Mouse Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit

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Specification

Product name Mouse Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGS1 in samples. An antibody specific for PTGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTGS1; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 1

Product Properties

Kit components • Mouse Prostaglandin G/H synthase 1 microplate
• Mouse Prostaglandin G/H synthase 1 standard
• Mouse Prostaglandin G/H synthase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTGS1. No significant cross-reactivity or interference between Mouse PTGS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Recent research has shown that the inhibition of COX-1 is the main reason why aspirin is effective at reducing cardiac events (as opposed to aspirin's anti-platelet aggregation effects). PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants called COX-1 and COX-3. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors.
Gene ID 19224
Alternative PTGS1; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 1
Accession P22437

Image & description

Fig.1. Mouse Prostaglandin G/H synthase 1 (PTGS1) Standard Curve.

Fig.1. Mouse Prostaglandin G/H synthase 1 (PTGS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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