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Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit

Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit

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Specification

Product name Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP1 in samples. An antibody specific for LRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LRP1; A2MR; APOER; APR; CD91; FLJ16451; IGFBP3R; LRP; MGC88725; TGFBR5; TbetaR-V/LRP-1/IGFBP-3 receptor; alpha-2-macroglobulin receptor; low density lipoprotein-related protein 1; type V tgf-beta receptor

Product Properties

Kit components • Mouse Prolow-density lipoprotein receptor-related protein 1 microplate
• Mouse Prolow-density lipoprotein receptor-related protein 1 standard
• Mouse Prolow-density lipoprotein receptor-related protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LRP1. No significant cross-reactivity or interference between Mouse LRP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Low density lipoprotein receptor-related protein 1 (alpha-2-macroglobulin receptor), also known as LRP1 or CD91, is a protein forming a receptor found in the plasma membrane of human cells involved in receptor-mediated endocytosis.Neurons require cholesterol to function. Cholesterol is imported into the neuron by the apolipoprotein apoE4 via LRP1 receptors on the cell surface. It is thought that a causal factor in Alzheimer's is the malfunction of this process which damages neurons by starving them of cholesterol.The protein showed strong calcium binding. Kristensen et al. (1990) and Strickland et al. (1990) demonstrated that LRP is identical to the alpha-2-macroglobulin (A2M) receptor (A2MR). Like the mannose-6-phosphate receptor, the A2MR/LRP molecule is probably bifunctional.
Gene ID 16971
Alternative LRP1; A2MR; APOER; APR; CD91; FLJ16451; IGFBP3R; LRP; MGC88725; TGFBR5; TbetaR-V/LRP-1/IGFBP-3 receptor; alpha-2-macroglobulin receptor; low density lipoprotein-related protein 1; type V tgf-beta receptor
Accession Q91ZX7

Image & description

Fig.1. Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) Standard Curve.

Fig.1. Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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