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Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit

Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit

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Specification

Product name Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6

Product Properties

Kit components • Mouse Proline dehydrogenase 1, mitochondrial microplate
• Mouse Proline dehydrogenase 1, mitochondrial standard
• Mouse Proline dehydrogenase 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRODH. No significant cross-reactivity or interference between Mouse PRODH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PRODH gene provides instructions for producing the enzyme proline oxidase (also known as proline dehydrogenase), which is found primarily in the brain, liver, and kidney. Within cells of these organs, this enzyme functions in energy-producing structures called mitochondria. Proline oxidase begins the process of breaking down the protein building block (amino acid) proline by starting the reaction that converts it to pyrroline-5-carboxylate. A subsequent step converts this intermediate product to the amino acid glutamate. The conversion between proline and glutamate is important in maintaining a supply of the amino acids needed for protein production, and for energy transfer within the cell.
Gene ID 19125
Alternative PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6
Accession Q9WU79

Image & description

Fig.1. Mouse Proline dehydrogenase 1, mitochondrial (PRODH) Standard Curve.

Fig.1. Mouse Proline dehydrogenase 1, mitochondrial (PRODH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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