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Mouse Proliferating cell nuclear antigen (PCNA) ELISA Kit

Mouse Proliferating cell nuclear antigen (PCNA) ELISA Kit

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Specification

Product name Mouse Proliferating cell nuclear antigen (PCNA) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Proliferating cell nuclear antigen (PCNA) ELISA Kit employs a two-site sandwich ELISA to quantitate PCNA in samples. An antibody specific for PCNA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCNA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCNA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCNA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCNA; MGC8367; DNA polymerase delta auxiliary protein; cyclin

Product Properties

Kit components • Mouse Proliferating cell nuclear antigen microplate
• Mouse Proliferating cell nuclear antigen standard
• Mouse Proliferating cell nuclear antigen detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Proliferating cell nuclear antigen (PCNA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PCNA. No significant cross-reactivity or interference between Mouse PCNA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proliferating Cell Nuclear Antigen a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. It achieves this processivity by encircling the DNA, thus creating a topological link to the genome. It is an example of a DNA clamp. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Gene ID 18538
Alternative PCNA; MGC8367; DNA polymerase delta auxiliary protein; cyclin
Accession P17918

Image & description

Fig.1. Mouse Proliferating cell nuclear antigen (PCNA) Standard Curve.

Fig.1. Mouse Proliferating cell nuclear antigen (PCNA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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