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Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit

Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit

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Specification

Product name Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLOD1 in samples. An antibody specific for PLOD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLOD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLOD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLOD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PLOD1; RP5-1077B9.2; FLJ42041; LH; LH1; LLH; PLOD; lysine hydroxylase; lysyl hydroxylase 1; procollagen-lysine; 2-oxoglutarate 5-dioxygenase

Product Properties

Kit components • Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 microplate
• Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 standard
• Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLOD1. No significant cross-reactivity or interference between Mouse PLOD1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PLOD1 encode a polypeptide of 709 amino acid residues and a signal peptide of 18 amino acids. The human coding sequence shows 72% identity to the chick sequences at the nucleotide level and 76% identity at the amino acid level. The C-terminal region is especially well conserved; residues 588 to 727 show 94% identity between the 2 species and residues 639 to 715 show 99% identity. The gene for lysyl hydroxylase, symbolized PLOD (for procollagen-lysine, 2-oxoglutarate 5-dioxygenase), was mapped to chromosome 1 by Southern blot analysis of human/mouse somatic cell hybrids, to the 1pter-1p34 region by use of cell hybrids that contained various rearrangements of human chromosome 1, and to 1p36.3-p36.2 by in situ hybridization.
Gene ID 18822
Alternative PLOD1; RP5-1077B9.2; FLJ42041; LH; LH1; LLH; PLOD; lysine hydroxylase; lysyl hydroxylase 1; procollagen-lysine; 2-oxoglutarate 5-dioxygenase
Accession Q9R0E2

Image & description

Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) Standard Curve.

Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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