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Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) ELISA Kit

Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) ELISA Kit

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Specification

Product name Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) ELISA Kit employs a two-site sandwich ELISA to quantitate PCOLCE in samples. An antibody specific for PCOLCE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCOLCE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCOLCE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCOLCE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCOLCE; PCPE; PCPE1; procollagen C-proteinase enhancer 1; procollagen; type 1; COOH-terminal proteinase enhancer

Product Properties

Kit components • Mouse Procollagen C-endopeptidase enhancer 1 microplate
• Mouse Procollagen C-endopeptidase enhancer 1 standard
• Mouse Procollagen C-endopeptidase enhancer 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PCOLCE. No significant cross-reactivity or interference between Mouse PCOLCE and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. In the mouse, procollagen C-proteinase depends for full expression upon the presence of either a 55-kD glycoprotein or smaller 36- and 34-kD proteolytically processed forms of the same protein. None of the 3 enhancer proteins exhibits intrinsic procollagen processing activity, but all are capable of enhancing the activity of the C-proteinase by approximately one order of magnitude. type I procollagen COOH-terminal proteinase enhancer, a glycoprotein that binds to the COOH-terminal propeptide of type I procollagen and enhances C-proteinase activity, from mouse fibroblast culture medium.
Gene ID 18542
Alternative PCOLCE; PCPE; PCPE1; procollagen C-proteinase enhancer 1; procollagen; type 1; COOH-terminal proteinase enhancer
Accession Q61398

Image & description

Fig.1. Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) Standard Curve.

Fig.1. Mouse Procollagen C-endopeptidase enhancer 1 (PCOLCE) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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