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Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit

Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit

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Specification

Product name Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit employs a two-site sandwich ELISA to quantitate POU5F1 in samples. An antibody specific for POU5F1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOU5F1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POU5F1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POU5F1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POU5F1; DADB-104B20.2; MGC22487; OCT3; OCT4; OTF3; OTF4; POU domain; class 5; transcription factor 1; POU-type homeodomain-containing DNA-binding protein; octamer-binding transcription factor-3

Product Properties

Kit components • Mouse POU domain, class 5, transcription factor 1 microplate
• Mouse POU domain, class 5, transcription factor 1 standard
• Mouse POU domain, class 5, transcription factor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POU5F1. No significant cross-reactivity or interference between Mouse POU5F1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Oct-4 is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated; too much or too little will actually cause differentiation of the cells. Oct-4 transcription factor is initially active as a maternal factor in the oocyte but remains active in embryos throughout the preimplantation period. Oct-4 expression is associated with an undifferentiated phenotype and tumors. In fact gene knockdown of Oct-4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Gene ID 18999
Alternative POU5F1; DADB-104B20.2; MGC22487; OCT3; OCT4; OTF3; OTF4; POU domain; class 5; transcription factor 1; POU-type homeodomain-containing DNA-binding protein; octamer-binding transcription factor-3
Accession P20263

Image & description

Fig.1. Mouse POU domain, class 5, transcription factor 1 (POU5F1) Standard Curve.

Fig.1. Mouse POU domain, class 5, transcription factor 1 (POU5F1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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