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Mouse Polycystin-1 (PKD1) ELISA Kit

Mouse Polycystin-1 (PKD1) ELISA Kit

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Specification

Product name Mouse Polycystin-1 (PKD1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Polycystin-1 (PKD1) ELISA Kit employs a two-site sandwich ELISA to quantitate PKD1 in samples. An antibody specific for PKD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PKD1; PBP; Pc-1; TRPP1; polycystic kidney disease-associated protein; polycystin 1; transient receptor potential cation channel; subfamily P; member 1

Product Properties

Kit components • Mouse Polycystin-1 microplate
• Mouse Polycystin-1 standard
• Mouse Polycystin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Polycystin-1 (PKD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PKD1. No significant cross-reactivity or interference between Mouse PKD1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Polycystin-1 is a protein that in humans is encoded by the PKD1 gene.Splice variants encoding different isoforms have been noted for PKD1. The gene is closely linked to six pseudogenes in a known duplicated region on chromosome 16p.Polycystin-1 is a glycoprotein which contains a large N-terminal extracellular region, multiple transmembrane domains and a cytoplasmic C-tail. It may function as an integral membrane protein involved in cell-cell/matrix interactions, and may modulate intracellular calcium homoeostasis and other signal-transduction pathways. It plays a role in renal tubular development, and mutations in this gene have been associated with autosomal dominant polycystic kidney disease.
Gene ID 18763
Alternative PKD1; PBP; Pc-1; TRPP1; polycystic kidney disease-associated protein; polycystin 1; transient receptor potential cation channel; subfamily P; member 1
Accession O08852

Image & description

Fig.1. Mouse Polycystin-1 (PKD1) Standard Curve.

Fig.1. Mouse Polycystin-1 (PKD1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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