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Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit

Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit

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Specification

Product name Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit employs a two-site sandwich ELISA to quantitate POGK in samples. An antibody specific for POGK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOGK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POGK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POGK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POGK; BASS2; KIAA1513; KIAA15131; LST003; OTTHUMP00000032216

Product Properties

Kit components • Mouse Pogo transposable element with KRAB domain microplate
• Mouse Pogo transposable element with KRAB domain standard
• Mouse Pogo transposable element with KRAB domain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POGK. No significant cross-reactivity or interference between Mouse POGK and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The exact function of the protein encoded by POGK is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs) . It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box.
Gene ID 71592
Alternative POGK; BASS2; KIAA1513; KIAA15131; LST003; OTTHUMP00000032216
Accession Q80TC5

Image & description

Fig.1. Mouse Pogo transposable element with KRAB domain (POGK) Standard Curve.

Fig.1. Mouse Pogo transposable element with KRAB domain (POGK) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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