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Mouse Podocan-like protein 1 (PODNL1) ELISA Kit

Mouse Podocan-like protein 1 (PODNL1) ELISA Kit

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Specification

Product name Mouse Podocan-like protein 1 (PODNL1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Podocan-like protein 1 (PODNL1) ELISA Kit employs a two-site sandwich ELISA to quantitate PODNL1 in samples. An antibody specific for PODNL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPODNL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PODNL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PODNL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PODNL1; FLJ23447; SLRR5B;

Product Properties

Kit components • Mouse Podocan-like protein 1 microplate
• Mouse Podocan-like protein 1 standard
• Mouse Podocan-like protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Podocan-like protein 1 (PODNL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PODNL1. No significant cross-reactivity or interference between Mouse PODNL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PODNL1, Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily. Studies of mice lacking specific members of the small leucine rich repeat proteoglycan (SLRP) family show that many of these connective tissue components have a role in the regulation of the structure and mechanical properties of collagen scaffold. Podocan is a secreted glycoprotein belonging to the small leucine-rich repeat (SLR) protein family. It lacks a serine/glycine dipeptide consensus sequence required for glycosaminoglycan attachment and is not a proteoglycan. Podocan transcripts were originally isolated from kidney podocytes. Podocan is also expressed in multiple other tissues including pineal gland, bladder, and vascular tissues. Podocan binds type I collagen and may affect cell migration.
Gene ID 244550
Alternative PODNL1; FLJ23447; SLRR5B;
Accession Q6P3Y9

Image & description

Fig.1. Mouse Podocan-like protein 1 (PODNL1) Standard Curve.

Fig.1. Mouse Podocan-like protein 1 (PODNL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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