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Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit

Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit

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Specification

Product name Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAA in samples. An antibody specific for PLAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PLAA; DOA1; FLJ11281; FLJ12699; PLA2P; PLAP; DOA1 homolog; phospholipase A2 activating protein

Product Properties

Kit components • Mouse Phospholipase A-2-activating protein microplate
• Mouse Phospholipase A-2-activating protein standard
• Mouse Phospholipase A-2-activating protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLAA. No significant cross-reactivity or interference between Mouse PLAA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phospholipase A2-activating protein (PLAP) is potentially important in regulating the inflammatory response through its activation of phospholipase A2, which catalyzes the release of arachidonic acid. By screening a human monocyte cDNA library with a mouse Plap cDNA, Chopra et al. (1999) isolated human PLAP cDNAs. The 738-amino acid human PLAP protein predicted by the cDNA sequence has a molecular mass of 80,826 kD; however, immunoblot analysis using antibodies against PLAP detected a 72- to 74-kD protein in human monocyte cell lysates. In mouse macrophages, an antisense Plap oligonucleotide blocked cholera toxin-induced arachidonic acid release, indicating a role for PLAP in the regulation of phospholipase A2 activation.
Gene ID 18786
Alternative PLAA; DOA1; FLJ11281; FLJ12699; PLA2P; PLAP; DOA1 homolog; phospholipase A2 activating protein
Accession P27612

Image & description

Fig.1. Mouse Phospholipase A-2-activating protein (PLAA) Standard Curve.

Fig.1. Mouse Phospholipase A-2-activating protein (PLAA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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