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Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit

Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit

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Specification

Product name Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3AP1 in samples. An antibody specific for PIK3AP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3AP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3AP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3AP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIK3AP1; BCAP; RP11-34E5.3; 1810044J04Rik; B cell adaptor protein

Product Properties

Kit components • Mouse Phosphoinositide 3-kinase adapter protein 1 microplate
• Mouse Phosphoinositide 3-kinase adapter protein 1 standard
• Mouse Phosphoinositide 3-kinase adapter protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3AP1. No significant cross-reactivity or interference between Mouse PIK3AP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Using an affinity purification strategy with the N-terminal SH2 domain of the phosphoinositide 3-kinase (PI3K) p85 subunit and anti-phosphotyrosine antibody, followed by screening a chicken DT40 B-cell cDNA library and a mouse spleen cDNA library, Okada et al. (2000) isolated cDNAs encoding chicken and mouse Pik3ap1, which they called Bcap. They also identified Bcap splice variants. Northern blot analysis of mouse tissues detected expression predominantly in spleen, with lower levels in thymus, liver, and lung. Expression was also detected in mouse macrophage and B-cell lines, but not in T-cell, plasma cell, or mast cell lines.Using mouse and chicken B- cell lines, Okada et al. (2000) determined that Bcap is a protein tyrosine kinase substrate that couples B-cell receptor to PI3K activation.
Gene ID 83490
Alternative PIK3AP1; BCAP; RP11-34E5.3; 1810044J04Rik; B cell adaptor protein
Accession Q9EQ32

Image & description

Fig.1. Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) Standard Curve.

Fig.1. Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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