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Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit

Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit

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Specification

Product name Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3CD in samples. An antibody specific for PIK3CD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3CD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIK3CD; P110DELTA; PI3K; p110D; PI3-kinase p110 subunit delta; catalytic phosphatidylinositol 3-kinase delta; phosphatidylinositol-4;5-bisphosphate 3-kinase catalytic subunit delta; phosphoinositide-3-kinase C

Product Properties

Kit components • Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform microplate
• Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform standard
• Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3CD. No significant cross-reactivity or interference between Mouse PIK3CD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha, p110-beta, and p110-gamma . p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras. Like p110-alpha and p110-beta, p110-delta binds p85 adaptor proteins and GTP-bound Ras. These 3 class I PI3Ks were indistinguishable at the level of p85 adaptor protein selection or recruitment to activated receptor complexes. However, unlike p110-alpha, p110-delta does not phosphorylate p85, but instead has an autophosphorylation activity.
Gene ID 18707
Alternative PIK3CD; P110DELTA; PI3K; p110D; PI3-kinase p110 subunit delta; catalytic phosphatidylinositol 3-kinase delta; phosphatidylinositol-4;5-bisphosphate 3-kinase catalytic subunit delta; phosphoinositide-3-kinase C
Accession O35904

Image & description

Fig.1. Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) Standard Curve.

Fig.1. Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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