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Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit

Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit

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Specification

Product name Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R2 in samples. An antibody specific for PIK3R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIK3R2; P85B; p85; p85-BETA; phosphatidylinositol 3-kinase; regulatory subunit; polypeptide 2 (p85 beta); phosphoinositide-3-kinase; regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase; regulatory subu

Product Properties

Kit components • Mouse Phosphatidylinositol 3-kinase regulatory subunit beta microplate
• Mouse Phosphatidylinositol 3-kinase regulatory subunit beta standard
• Mouse Phosphatidylinositol 3-kinase regulatory subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3R2. No significant cross-reactivity or interference between Mouse PIK3R2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3-prime position. The products of these reactions serve as second messengers in growth signaling pathways. The kinase itself is made up of a catalytic subunit of molecular mass 110 kD (PIK3CA) and a regulatory subunit of either 85 kD, 55 kD, or 50 kD. Both p85-alpha and p85-beta bound Xbp1s and increased its nuclear translocation, and it appeared that the p110 PI3K catalytic subunit and Xbp1s competed for binding of these regulatory subunits. p85-alpha and p85-beta formed an inactive dimer that was disrupted by insulin in a time-dependent manner, which promoted their association with Xbp1s.
Gene ID 18709
Alternative PIK3R2; P85B; p85; p85-BETA; phosphatidylinositol 3-kinase; regulatory subunit; polypeptide 2 (p85 beta); phosphoinositide-3-kinase; regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase; regulatory subu
Accession O08908

Image & description

Fig.1. Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) Standard Curve.

Fig.1. Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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