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Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit

Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit

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Specification

Product name Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX6 in samples. An antibody specific for PRDX6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRDX6; 1-Cys; AOP2; KIAA0106; MGC46173; NSGPx; PRX; aiPLA2; p29; 1-Cys peroxiredoxin; acidic calcium-independent phospholipase A2; antioxidant protein 2; non-selenium glutathione peroxidase

Product Properties

Kit components • Mouse Peroxiredoxin-6 microplate
• Mouse Peroxiredoxin-6 standard
• Mouse Peroxiredoxin-6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRDX6. No significant cross-reactivity or interference between Mouse PRDX6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Kim et al. (1997) isolated a calcium-independent lysosomal PLA2 from a human myeloblast cell line. The predicted 224-amino acid protein contained the PLA2 catalytic motif, but no other significant homology to known phospholipases. Expressed protein had significant PLA2 activity on several substrates.
Gene ID 11758
Alternative PRDX6; 1-Cys; AOP2; KIAA0106; MGC46173; NSGPx; PRX; aiPLA2; p29; 1-Cys peroxiredoxin; acidic calcium-independent phospholipase A2; antioxidant protein 2; non-selenium glutathione peroxidase
Accession O08709

Image & description

Fig.1. Mouse Peroxiredoxin-6 (PRDX6) Standard Curve.

Fig.1. Mouse Peroxiredoxin-6 (PRDX6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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