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Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit

Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit

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Specification

Product name Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit employs a two-site sandwich ELISA to quantitate PPIC in samples. An antibody specific for PPIC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPIC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPIC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPIC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PPIC; CYPC; MGC3673; PPIase C; cyclophilin C; parvulin; peptidyl-prolyl cis-trans isomerase C; peptidylprolyl isomerase C; rotamase C

Product Properties

Kit components • Mouse Peptidyl-prolyl cis-trans isomerase C microplate
• Mouse Peptidyl-prolyl cis-trans isomerase C standard
• Mouse Peptidyl-prolyl cis-trans isomerase C detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPIC. No significant cross-reactivity or interference between Mouse PPIC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Peptidyl-prolyl cis-trans isomerase C is a member of the peptidyl-prolyl cis-trans isomerase (PPIase)) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A. Friedman and Weissman (1991) cloned cyclophilin C from a cDNA library prepared from a murine bone marrow-derived stromal cell line. Their initial interest in the cDNA was based on its high level of homology with all known cyclophilins. The tissue distribution made it a candidate for the mediator of the immunosuppressive and nephrotoxic actions of cyclosporin A, as it is expressed most highly in the kidney and can be detected in activated T cells.
Gene ID 19038
Alternative PPIC; CYPC; MGC3673; PPIase C; cyclophilin C; parvulin; peptidyl-prolyl cis-trans isomerase C; peptidylprolyl isomerase C; rotamase C
Accession P30412

Image & description

Fig.1. Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) Standard Curve.

Fig.1. Mouse Peptidyl-prolyl cis-trans isomerase C (PPIC) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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