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Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit

Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit

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Specification

Product name Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1L in samples. An antibody specific for MTRF1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRF1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MTRF1L; RP1-101K10.1; MGC102748; MRF1L; mtRF1a; mitochondrial release factor 1 like

Product Properties

Kit components • Mouse Peptide chain release factor 1-like, mitochondrial microplate
• Mouse Peptide chain release factor 1-like, mitochondrial standard
• Mouse Peptide chain release factor 1-like, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MTRF1L. No significant cross-reactivity or interference between Mouse MTRF1L and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Mitochondrial Translational Release Factor 1 Like encoded by MTRF1L plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. This protein acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. This protein shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants.
Gene ID 108853
Alternative MTRF1L; RP1-101K10.1; MGC102748; MRF1L; mtRF1a; mitochondrial release factor 1 like
Accession Q8BJU9

Image & description

Fig.1. Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) Standard Curve.

Fig.1. Mouse Peptide chain release factor 1-like, mitochondrial (MTRF1L) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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