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Mouse Paraneoplastic antigen Ma1 (PNMA1) ELISA Kit

Mouse Paraneoplastic antigen Ma1 (PNMA1) ELISA Kit

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Specification

Product name Mouse Paraneoplastic antigen Ma1 (PNMA1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Paraneoplastic antigen Ma1 (PNMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PNMA1 in samples. An antibody specific for PNMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PNMA1; MA1; neuron- and testis-specific protein 1; paraneoplastic neuronal antigen MA1

Product Properties

Kit components • Mouse Paraneoplastic antigen Ma1 microplate
• Mouse Paraneoplastic antigen Ma1 standard
• Mouse Paraneoplastic antigen Ma1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Paraneoplastic antigen Ma1 (PNMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PNMA1. No significant cross-reactivity or interference between Mouse PNMA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PNMA1 gene encodes an antineuronal antibody (anti-Ma) present in patients with paraneoplastic neurologic disorders). Some paraneoplastic syndromes affecting the nervous system are associated with antibodies that react with neuronal proteins and the causal tumor. Several of these antibodies are markers of specific neurologic syndromes associated with distinct types of cancer. The presence of some antibodies is so specific that disorders previously identified by brain biopsy or at autopsy can be diagnosed serologically. The expression of neuronal proteins by the tumor appears to be a crucial step that breaks the immune tolerance for otherwise normal neuronal proteins. The identity of most onconeuronal antigens was established by probing human cDNA expression libraries with serum containing antineuronal antibodies.
Gene ID 70481
Alternative PNMA1; MA1; neuron- and testis-specific protein 1; paraneoplastic neuronal antigen MA1
Accession Q8C1C8

Image & description

Fig.1. Mouse Paraneoplastic antigen Ma1 (PNMA1) Standard Curve.

Fig.1. Mouse Paraneoplastic antigen Ma1 (PNMA1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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