|Product name||Mouse Oxidizided glutathione (GSSG) ELISA Kit|
|Applications notes||This Mouse Oxidizided glutathione (GSSG) ELISA Kit employs a two-site sandwich ELISA to quantitate GSSG in samples. An antibody specific for GSSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSSG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSSG bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Oxidizided glutathione microplate
• Mouse Oxidizided glutathione standard
• Mouse Oxidizided glutathione detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Oxidizided glutathione (GSSG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GSSG. No significant cross-reactivity or interference between Mouse GSSG and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Oxidized glutathione represents one percent of the total cellular glutathione (GSH) concentration. Glutathione, the most abundant intracellular non-protein thiol, is formed in cells as a means of protecting against hydroperoxides. As is well known, peroxides are unstable and, upon decomposition, form very deadly free radicals. Glutathione scavenges these free radicals using the thiol bridge (-SH groups), which generates water and yields the oxidized form of glutathione.The appropriate name for glutathione is N(N-y-L-glutamyl-L-cysteinyl-glycine). The easiest way of describing this compound is to envision two identical molecules connected to each other by a thiol bridge. The identical molecules consist of glutamic acid connected through the gamma carbon carboxylic acid terminal to the amino group of the amino acid cysteine, which is connected to the amino group of the amino acid glycine.|
Fig.1. Mouse Oxidizided glutathione (GSSG) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.