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Mouse Nuclear receptor coactivator 5 (NCOA5) ELISA Kit

Mouse Nuclear receptor coactivator 5 (NCOA5) ELISA Kit

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Specification

Product name Mouse Nuclear receptor coactivator 5 (NCOA5) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Nuclear receptor coactivator 5 (NCOA5) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA5 in samples. An antibody specific for NCOA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NCOA5; RP11-465L10.6; CIA; bA465L10.6; coactivator independent of AF-2

Product Properties

Kit components • Mouse Nuclear receptor coactivator 5 microplate
• Mouse Nuclear receptor coactivator 5 standard
• Mouse Nuclear receptor coactivator 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Nuclear receptor coactivator 5 (NCOA5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCOA5. No significant cross-reactivity or interference between Mouse NCOA5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ncoa5 encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The nuclear receptor coactivator 5 localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Two alternatively spliced transcript variants for this gene have been described. However, the full length nature of one of the variants has not been determined.
Gene ID 228869
Alternative NCOA5; RP11-465L10.6; CIA; bA465L10.6; coactivator independent of AF-2
Accession Q91W39

Image & description

Fig.1. Mouse Nuclear receptor coactivator 5 (NCOA5) Standard Curve.

Fig.1. Mouse Nuclear receptor coactivator 5 (NCOA5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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