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Mouse Nuclear receptor coactivator 2 (NCOA2) ELISA Kit

Mouse Nuclear receptor coactivator 2 (NCOA2) ELISA Kit

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Specification

Product name Mouse Nuclear receptor coactivator 2 (NCOA2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Nuclear receptor coactivator 2 (NCOA2) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA2 in samples. An antibody specific for NCOA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NCOA2; GRIP1; KAT13C; MGC138808; NCoA-2; SRC2; TIF2; bHLHe75; glucocorticoid receptor-interacting protein-1

Product Properties

Kit components • Mouse Nuclear receptor coactivator 2 microplate
• Mouse Nuclear receptor coactivator 2 standard
• Mouse Nuclear receptor coactivator 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Nuclear receptor coactivator 2 (NCOA2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCOA2. No significant cross-reactivity or interference between Mouse NCOA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The nuclear receptor coactivator 2 encoded by Ncoa2 functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants.
Gene ID 17978
Alternative NCOA2; GRIP1; KAT13C; MGC138808; NCoA-2; SRC2; TIF2; bHLHe75; glucocorticoid receptor-interacting protein-1
Accession Q61026

Image & description

Fig.1. Mouse Nuclear receptor coactivator 2 (NCOA2) Standard Curve.

Fig.1. Mouse Nuclear receptor coactivator 2 (NCOA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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