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Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit

Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit

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Specification

Product name Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit employs a two-site sandwich ELISA to quantitate NFE2L3 in samples. An antibody specific for NFE2L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFE2L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFE2L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFE2L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NFE2L3; NRF3; nuclear factor erythroid 2-like 3; nuclear factor-erythroid 2 p45-related factor 3; nuclear factor-erythroid 2-related factor 3

Product Properties

Kit components • Mouse Nuclear factor erythroid 2-related factor 3 microplate
• Mouse Nuclear factor erythroid 2-related factor 3 standard
• Mouse Nuclear factor erythroid 2-related factor 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NFE2L3. No significant cross-reactivity or interference between Mouse NFE2L3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NFE2-binding sites and Maf recognition elements (MAREs) are essential cis-acting elements in the regulatory regions of erythroid-specific genes recognized by the erythroid transcription factor NFE2, which is composed of p45 and a small MAF protein. The p45 subunit and the p45-related factors NRF1 (NFE2L1) and NRF2 (NFE2L2) contain a Cap'n'collar (CNC)-type basic leucine zipper (bZIP) domain, and they are collectively grouped as the CNC family. The cDNA sequence encodes a novel 694-amino acid polypeptide, which the authors called NRF3. NRF3 contains a bZIP domain that is similar to those of other CNC transcription factors. Northern blot analysis detected 2 human NRF3 transcripts in all tissues examined, with the highest levels in placenta. NRF3 was expressed in B- and monocytic cell lines but not in T-cell lines.
Gene ID 18025
Alternative NFE2L3; NRF3; nuclear factor erythroid 2-like 3; nuclear factor-erythroid 2 p45-related factor 3; nuclear factor-erythroid 2-related factor 3
Accession Q9WTM4

Image & description

Fig.1. Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) Standard Curve.

Fig.1. Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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