Login Register
English
0

Cart

$ 0

Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit

Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit

Views(153) Publications(0) Catalog no(KTE71322)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit employs a two-site sandwich ELISA to quantitate DCX in samples. An antibody specific for DCX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DCX; RP5-914P14.1; DBCN; DC; LISX; SCLH; XLIS; doublecortex; doublin; lissencephalin-X

Product Properties

Kit components • Mouse Neuronal migration protein doublecortin microplate
• Mouse Neuronal migration protein doublecortin standard
• Mouse Neuronal migration protein doublecortin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DCX. No significant cross-reactivity or interference between Mouse DCX and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Doublecortin encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis, and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene.
Gene ID 13193
Alternative DCX; RP5-914P14.1; DBCN; DC; LISX; SCLH; XLIS; doublecortex; doublin; lissencephalin-X
Accession O88809

Image & description

Fig.1. Mouse Neuronal migration protein doublecortin (DCX) Standard Curve.

Fig.1. Mouse Neuronal migration protein doublecortin (DCX) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Neuronal migration protein doublecortin (DCX) ELISA Kit”