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Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) ELISA Kit

Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) ELISA Kit

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Specification

Product name Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) ELISA Kit employs a two-site sandwich ELISA to quantitate NET1 in samples. An antibody specific for NET1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNET1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NET1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NET1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NET1; RP11-116G8.1; ARHGEF8; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelial cell transforming gene 1; neuroepithelioma transforming g

Product Properties

Kit components • Mouse Neuroepithelial cell-transforming gene 1 protein microplate
• Mouse Neuroepithelial cell-transforming gene 1 protein standard
• Mouse Neuroepithelial cell-transforming gene 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NET1. No significant cross-reactivity or interference between Mouse NET1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The norepinephrine transporter or NET is a monoamine transporter that transports the neurotransmitter norepinephrine (noradrenaline) from the synapse back to its vesicles for storage until later use. It also appears to transport the neurotransmitter dopamine in the same way, but to a lesser degree. "The norepinephrine transporter (NET) protein consists of 617 amino acids and has 12 transmembrane domains" and is encoded by the SLC6A2 gene. Certain antidepressant medications act to raise noradrenaline, such as the SNRIs and the tricyclic antidepressants (TCAs). In other words, these medications prevent the noradrenaline transporter from doing its job. Noradrenaline therefore remains in the synapse longer, allowing it to reach more normal levels.
Gene ID 56349
Alternative NET1; RP11-116G8.1; ARHGEF8; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelial cell transforming gene 1; neuroepithelioma transforming g
Accession Q9Z206

Image & description

Fig.1. Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) Standard Curve.

Fig.1. Mouse Neuroepithelial cell-transforming gene 1 protein (NET1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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