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Mouse Neuregulin-1 (NRG1) ELISA Kit

Mouse Neuregulin-1 (NRG1) ELISA Kit

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Specification

Product name Mouse Neuregulin-1 (NRG1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Neuregulin-1 (NRG1) ELISA Kit employs a two-site sandwich ELISA to quantitate NRG1 in samples. An antibody specific for NRG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNRG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NRG1; ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; NDF; SMDF; glial growth factor; heregulin; alpha (45kD; ERBB2 p185-activator); neu differentiation factor; sensory and motor neuron derived factor

Product Properties

Kit components • Mouse Neuregulin-1 microplate
• Mouse Neuregulin-1 standard
• Mouse Neuregulin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Neuregulin-1 (NRG1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NRG1. No significant cross-reactivity or interference between Mouse NRG1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Neuregulin 1 or NRG1 is one of the four proteins of the neuregulin family which act on EGFR family of receptors. Neuregulin 1 is produced in numerous isoforms by alternative splicing, and this allows it to perform a wide variety of functions. Neuregulin 1-ErbB4 interactions are thought to play a role in the pathological mechanism of schizophrenia. The protein also has a putative ability to protect the brain from damage induced by stroke. Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing.
Alternative NRG1; ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; NDF; SMDF; glial growth factor; heregulin; alpha (45kD; ERBB2 p185-activator); neu differentiation factor; sensory and motor neuron derived factor

Image & description

Fig.1. Mouse Neuregulin-1 (NRG1) Standard Curve.

Fig.1. Mouse Neuregulin-1 (NRG1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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