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Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit

Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit

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Specification

Product name Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate NCAM1 in samples. An antibody specific for NCAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NCAM1; CD56; MSK39; NCAM; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule; NCAM

Product Properties

Kit components • Mouse Neural cell adhesion molecule 1 microplate
• Mouse Neural cell adhesion molecule 1 standard
• Mouse Neural cell adhesion molecule 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCAM1. No significant cross-reactivity or interference between Mouse NCAM1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NCAM1 encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The Neural Cell Adhesion Molecule 1 is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Gene ID 17967
Alternative NCAM1; CD56; MSK39; NCAM; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule; NCAM
Accession P13595

Image & description

Fig.1. Mouse Neural cell adhesion molecule 1 (NCAM1) Standard Curve.

Fig.1. Mouse Neural cell adhesion molecule 1 (NCAM1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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