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Mouse Neurabin-2 (PPP1R9B) ELISA Kit

Mouse Neurabin-2 (PPP1R9B) ELISA Kit

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Specification

Product name Mouse Neurabin-2 (PPP1R9B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Neurabin-2 (PPP1R9B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1R9B in samples. An antibody specific for PPP1R9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1R9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1R9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PPP1R9B; FLJ30345; PPP1R6; PPP1R9; SPINO; Spn; Neurabin-2; neurabin; protein phosphatase 1; regulatory subunit 9B; protein phosphatase 1; regulatory subunit 9B; spinophilin; spinophilin

Product Properties

Kit components • Mouse Neurabin-2 microplate
• Mouse Neurabin-2 standard
• Mouse Neurabin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Neurabin-2 (PPP1R9B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPP1R9B. No significant cross-reactivity or interference between Mouse PPP1R9B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system.To identify proteins that interact with the ARF protein encoded by the CDKN2A gene, Vivo et al. (2001) used a yeast 2-hybrid screen of a human brain cDNA library with an ARF fusion construct as bait. By database searching with the identified clones, they reconstructed a full-length cDNA of human spinophilin. The deduced 813-amino acid protein shares 95% sequence identity with the rat homolog and contains an F-actin binding domain, a PP1C binding site, a PDZ domain, and a myosin-like left-handed alpha helix.
Gene ID 217124
Alternative PPP1R9B; FLJ30345; PPP1R6; PPP1R9; SPINO; Spn; Neurabin-2; neurabin; protein phosphatase 1; regulatory subunit 9B; protein phosphatase 1; regulatory subunit 9B; spinophilin; spinophilin
Accession Q6R891

Image & description

Fig.1. Mouse Neurabin-2 (PPP1R9B) Standard Curve.

Fig.1. Mouse Neurabin-2 (PPP1R9B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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