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Mouse NADPH--cytochrome P450 reductase (POR) ELISA Kit

Mouse NADPH--cytochrome P450 reductase (POR) ELISA Kit

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Specification

Product name Mouse NADPH--cytochrome P450 reductase (POR) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse NADPH--cytochrome P450 reductase (POR) ELISA Kit employs a two-site sandwich ELISA to quantitate POR in samples. An antibody specific for POR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POR; CPR; CYPOR; DKFZp686G04235; FLJ26468; P450R; NADPH-dependent cytochrome P450 reductase; cytochrome P450 reductase

Product Properties

Kit components • Mouse NADPH--cytochrome P450 reductase microplate
• Mouse NADPH--cytochrome P450 reductase standard
• Mouse NADPH--cytochrome P450 reductase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse NADPH--cytochrome P450 reductase (POR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POR. No significant cross-reactivity or interference between Mouse POR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The POR gene provides instructions for making the enzyme cytochrome P450 oxidoreductase. This enzyme is required for the normal functioning of more than 50 enzymes in the cytochrome P450 family. Cytochrome P450 enzymes are involved in the formation (synthesis) and breakdown (metabolism) of various molecules and chemicals within cells. Cytochrome P450 enzymes are critical for the synthesis of cholesterol and steroid hormones. Cholesterol is a substance that has many essential functions both before and after birth, including roles in the production of steroid hormones and in the formation and growth of bones. Steroid hormones are needed for normal development and reproduction. This group of hormones includes testosterone and estrogen, which are essential for normal sexual development and reproduction; corticosteroids, which are involved in the body's response to stress; and aldosterone, which helps regulate the body's salt and water balance.
Gene ID 18984
Alternative POR; CPR; CYPOR; DKFZp686G04235; FLJ26468; P450R; NADPH-dependent cytochrome P450 reductase; cytochrome P450 reductase
Accession P37040

Image & description

Fig.1. Mouse NADPH--cytochrome P450 reductase (POR) Standard Curve.

Fig.1. Mouse NADPH--cytochrome P450 reductase (POR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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