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Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) ELISA Kit

Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) ELISA Kit

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Specification

Product name Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5R1 in samples. An antibody specific for CYB5R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYB5R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CYB5R1; B5R.1; B5R1; NQO3A2; humb5R2; NAD(P)H:quinone oxidoreductase type 3; polypeptide A2

Product Properties

Kit components • Mouse NADH-cytochrome b5 reductase 1 microplate
• Mouse NADH-cytochrome b5 reductase 1 standard
• Mouse NADH-cytochrome b5 reductase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYB5R1. No significant cross-reactivity or interference between Mouse CYB5R1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CYB5R1 (Cytochrome B5 Reductase 1) is a Protein Coding gene. Among its related pathways are Metabolism and Amino sugar and nucleotide sugar metabolism. GO annotations related to this gene include oxidoreductase activity and cytochrome-b5 reductase activity, acting on NAD(P)H. An important paralog of this gene is CYB5R3. NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Gene ID 72017
Alternative CYB5R1; B5R.1; B5R1; NQO3A2; humb5R2; NAD(P)H:quinone oxidoreductase type 3; polypeptide A2
Accession Q9DB73

Image & description

Fig.1. Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) Standard Curve.

Fig.1. Mouse NADH-cytochrome b5 reductase 1 (CYB5R1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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