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Mouse Myosin light chain 6B (MYL6B) ELISA Kit

Mouse Myosin light chain 6B (MYL6B) ELISA Kit

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Specification

Product name Mouse Myosin light chain 6B (MYL6B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Myosin light chain 6B (MYL6B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL6B in samples. An antibody specific for MYL6B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL6B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL6B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL6B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYL6B; MLC1SA; myosin alkali light chain 1 slow a; myosin light chain 1 slow a; myosin light chain 1; slow-twitch muscle A isoform; myosin; light polypeptide 6B; alkali; smooth muscle and non-muscle; smooth mu

Product Properties

Kit components • Mouse Myosin light chain 6B microplate
• Mouse Myosin light chain 6B standard
• Mouse Myosin light chain 6B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Myosin light chain 6B (MYL6B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYL6B. No significant cross-reactivity or interference between Mouse MYL6B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6B encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants.
Gene ID 216459
Alternative MYL6B; MLC1SA; myosin alkali light chain 1 slow a; myosin light chain 1 slow a; myosin light chain 1; slow-twitch muscle A isoform; myosin; light polypeptide 6B; alkali; smooth muscle and non-muscle; smooth mu
Accession Q8CI43

Image & description

Fig.1. Mouse Myosin light chain 6B (MYL6B) Standard Curve.

Fig.1. Mouse Myosin light chain 6B (MYL6B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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