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Mouse Myosin-IXb (MYO9B) ELISA Kit

Mouse Myosin-IXb (MYO9B) ELISA Kit

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Specification

Product name Mouse Myosin-IXb (MYO9B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Myosin-IXb (MYO9B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO9B in samples. An antibody specific for MYO9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYO9B; CELIAC4; MYR5; unconventional myosin IXb

Product Properties

Kit components • Mouse Myosin-IXb microplate
• Mouse Myosin-IXb standard
• Mouse Myosin-IXb detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Myosin-IXb (MYO9B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO9B. No significant cross-reactivity or interference between Mouse MYO9B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activator for RHOA. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA.
Alternative MYO9B; CELIAC4; MYR5; unconventional myosin IXb
Accession Q9QY06

Image & description

Fig.1. Mouse Myosin-IXb (MYO9B) Standard Curve.

Fig.1. Mouse Myosin-IXb (MYO9B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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