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Mouse Myosin-Ih (MYO1H) ELISA Kit

Mouse Myosin-Ih (MYO1H) ELISA Kit

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Specification

Product name Mouse Myosin-Ih (MYO1H) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Myosin-Ih (MYO1H) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1H in samples. An antibody specific for MYO1H has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1H present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1H is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1H bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYO1H; FLJ37587; myosin 1H

Product Properties

Kit components • Mouse Myosin-Ih microplate
• Mouse Myosin-Ih standard
• Mouse Myosin-Ih detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Myosin-Ih (MYO1H) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO1H. No significant cross-reactivity or interference between Mouse MYO1H and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity).
Alternative MYO1H; FLJ37587; myosin 1H
Accession Q9D6A1

Image & description

Fig.1. Mouse Myosin-Ih (MYO1H) Standard Curve.

Fig.1. Mouse Myosin-Ih (MYO1H) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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