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Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit

Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit

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Specification

Product name Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit employs a two-site sandwich ELISA to quantitate MAZ in samples. An antibody specific for MAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAZ; PUR1; Pur-1; SAF-1; SAF-2; SAF-3; ZF87; ZNF801; Zif87; MYC-associated zinc finger protein; purine-binding transcription factor; serum amyloid A activating factor 1; serum amyloid A activating factor 2;

Product Properties

Kit components • Mouse Myc-associated zinc finger protein microplate
• Mouse Myc-associated zinc finger protein standard
• Mouse Myc-associated zinc finger protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAZ. No significant cross-reactivity or interference between Mouse MAZ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The MYC gene product functions as a DNA-specific transcription factor. A related polypeptide called MAX , for MYC-associated factor X, dimerizes with MYC to optimize DNA binding. A paucity of negative regulatory elements have been reported for the MYC gene which regulate transcription from 2 initiation sites specified by the P1 and P2 promoters. ME1a1, a 16-bp nuclear nuclear factor binding site residing between MYC P1 and P2 transcription initiation sites, is required for P2 activity. MAZ, Its mRNA was present in all tissues tested (except kidney) as a doublet of approximately 2.5 to 2.7 kb, along with differentially expressed minor species. The authors speculated that MAZ may encode a transcription factor with dual roles in transcription initiation and termination.
Alternative MAZ; PUR1; Pur-1; SAF-1; SAF-2; SAF-3; ZF87; ZNF801; Zif87; MYC-associated zinc finger protein; purine-binding transcription factor; serum amyloid A activating factor 1; serum amyloid A activating factor 2;
Accession P56671

Image & description

Fig.1. Mouse Myc-associated zinc finger protein (MAZ) Standard Curve.

Fig.1. Mouse Myc-associated zinc finger protein (MAZ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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