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Mouse Multidrug resistance-associated protein 1 (ABCC1) ELISA Kit

Mouse Multidrug resistance-associated protein 1 (ABCC1) ELISA Kit

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Specification

Product name Mouse Multidrug resistance-associated protein 1 (ABCC1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Multidrug resistance-associated protein 1 (ABCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC1 in samples. An antibody specific for ABCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABCC1; ABC29; ABCC; DKFZp686N04233; DKFZp781G125; GS-X; MRP; MRP1; ATP-binding cassette; sub-family C; member 1; LTC4 transporter; leukotriene C(4) transporter; multidrug resistance protein; multiple drug resi

Product Properties

Kit components • Mouse Multidrug resistance-associated protein 1 microplate
• Mouse Multidrug resistance-associated protein 1 standard
• Mouse Multidrug resistance-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Multidrug resistance-associated protein 1 (ABCC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCC1. No significant cross-reactivity or interference between Mouse ABCC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene.The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms.
Gene ID 17250
Alternative ABCC1; ABC29; ABCC; DKFZp686N04233; DKFZp781G125; GS-X; MRP; MRP1; ATP-binding cassette; sub-family C; member 1; LTC4 transporter; leukotriene C(4) transporter; multidrug resistance protein; multiple drug resi
Accession O35379

Image & description

Fig.1. Mouse Multidrug resistance-associated protein 1 (ABCC1) Standard Curve.

Fig.1. Mouse Multidrug resistance-associated protein 1 (ABCC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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