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Mouse Monoclonal antibody isotyping ELISA Kit

Mouse Monoclonal antibody isotyping ELISA Kit

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Specification

Product name Mouse Monoclonal antibody isotyping ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Monoclonal antibody isotyping ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoclonal antibody isotyping. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Monoclonal antibody isotyping and incubated. The competitive inhibition reaction is launched between with HRP labeled Monoclonal antibody isotyping and unlabeled Monoclonal antibody isotyping with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Monoclonal antibody isotyping in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Monoclonal antibody isotyping

Product Properties

Kit components • Mouse Monoclonal antibody isotyping ELISA Kit microplate
• Mouse Monoclonal antibody isotyping ELISA Kit standard
• Mouse Monoclonal antibody isotyping ELISA Kit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Monoclonal antibody isotyping ELISA Kit has high sensitivity and excellent specificity for detection of Mouse Monoclonal antibody isotyping. No significant cross-reactivity or interference between Mouse Monoclonal antibody isotyping and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Monoclonal antibodies (mAb or moAb) are antibodies that are made by identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope (the part of anantigen that is recognized by the antibody). In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different plasma cell (antibody secreting immune cell) lineages. Bispecific monoclonal antibodies can also be engineered, by increasing the therapeutic targets of one single monoclonal antibody to two epitopes.
Alternative Monoclonal antibody isotyping

Image & description

Fig.1. Mouse Monoclonal antibody isotyping Standard Curve.

Fig.1. Mouse Monoclonal antibody isotyping Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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